Training for digital preservation in the context of the European project PLANETS

This paper outlines a training programme jointly developed and organised by the Humanities Advanced Technology and Information Institute (‘HATII’) at the University of Glasgow and the British Library, in collaboration with a number of European partner institutions, on behalf of the Preservation and Long Term Access Through Networked Services (‘Planets’) project. It describes the background to the programme and the series of events which took place during the final year of the project, focussing on the feedback received from the participants, the lessons learned from the implementation of the events, and the perceived long-term impact of the programme on future digital preservation training activities

Training for digital preservation in the context of the European project PLANETS

This paper outlines a training programme jointly developed and organised by the Humanities Advanced Technology and Information Institute (‘HATII’) at the University of Glasgow and the British Library, in collaboration with a number of European partner institutions, on behalf of the Preservation and Long Term Access Through Networked Services (‘Planets’) project. It describes the background to the programme and the series of events which took place during the final year of the project, focussing on the feedback received from the participants, the lessons learned from the implementation of the events, and the perceived long-term impact of the programme on future digital preservation training activities

The integration of human factors, operability and personnel movement simulation into the preliminary design of ships utilising the Design Building Block approach

This thesis presents the feasibility, advantages and impact on Preliminary Ship Design of an approach to integrate ship configurational design with the modelling and simulation of a range of crewing issues, such as operations and evacuation. Integrating personnel movement simulation into preliminary ship design introduces the assessment of onboard operations at the front-end of the design process, informing the design and enabling improved operability while the design is still amenable to changes. The approach to accomplish this integration is discussed with the aim of informing all parties involved in the design of ships with regard to the main aspects of personnel operability and on board safety. The research was undertaken as part of a three years research project funded by the Engineering and Physical Sciences Research Council (EPSRC) entitled “Guidance on the Design of Ships for Enhanced Escape and Operation”. The project aimed at bringing together the University of Greenwich developed “maritimeEXODUS” personnel movement simulation software and the SURFCON implementation in the PARAMARINE suite of the Design Building Block approach to Preliminary Ship Design, which originated with the UCL Ship Design Research team. The approach and procedural implications of integrating personnel movement simulation into the preliminary ship design process are presented through a series of SURFCON ship design case studies. With the UK Ministry of Defence as the industrial partner to the project, this study on “design for operation” concentrates on naval vessels, which provide excellent examples of complex environments. Design studies, based on the Royal Navy Type 22 Batch III Frigate design, were analysed using PARAMARINE, maritimeEXODUS and bespoke interface software produced by the candidate. Technical aspects of the development of the interface software are discussed from a procedural perspective, focusing on integration and usability issues. The discussion addresses alternative options to visualising the simulation results and how to integrate into a ship design model a minimum level of detail sufficient to conduct simulations able to inform the designer, while retaining the flexibility the design requires in early stages design. The thesis concludes by summarising the opportunities that integrating operational simulation into preliminary ship design opens up for the future practice of ship design, contributing to the debate on the nature of ship design and of Computer Aided Preliminary Ship Design

Sorghum used to fodder production in dry farming

In Italy water deficient increase forward to cultivate resistant crops for forage production. In the present research it has been studied the opportunity of using 2 varieties of sorghum: the "Sweet Creek", used as green forage and for silage and the "True", with thinner stalks, used as hay. The fodder production and the dhurrin content during the vegetative phase of the 2 varieties were recorded. Production and chemical characteristics of green and preserved fodders (hay and silage) were determined; moreover the nutritive value and the in vitro digestibility of DM were measured. Results confirm the good adaptation of the sorghum to the water limited conditions as those ones in which the test has been carried out; green and preserved fodders yield were high, however during the hay harvest problems due to the different drying dynamics of leaves and stalks were found. The dhurrin content of these two varieties, even in the young phase, allows the use for grazing of the regrown, which have good bunching

Nutraceutical and technological properties of buffalo and sheep cheese produced by the addition of kiwi juice as a coagulant

Kiwifruit is an interesting alternative to chymosin for milk coagulation. Although the clotting properties of actinidin (the proteolytic agent present in kiwi) have been widely investigated, little is known about the nutraceutical and organoleptic effects of kiwifruit on the characteristics of cheese. We investigated kiwifruit pulp, compared to calf rennet, in cheesemaking using sheep and buffalo milk. Although the kiwifruit extract showed a longer coagulation and syneresis time than calf rennet, it could nevertheless be exploited as a plant coagulant due to its positive effect on the nutraceutical properties. In fact, the sheep and buffalo cheese were higher in polyphenols and phytosterols than the cheese obtained using calf rennet. In addition, the nutraceutical properties were enhanced, with just a slight effect on the aroma of the cheese

Compensatory Feto-Placental Upregulation of the Nitric Oxide System during Fetal Growth Restriction

Background: Fetal Growth Restriction is often associated with a feto-placental vascular dysfunction conceivably involving endothelial cells. Our study aimed to verify this pathogenic role for feto-placental endothelial cells and, coincidentally, demonstrate any abnormality in the nitric oxide system. Methods: Prenatal assessment of feto-placental vascular function was combined with measurement of nitric oxide (in the form of S-nitrosohemoglobin) and its nitrite byproduct, and of the endogenous nitric oxide synthase inhibitor asymmetric dimethylarginine. Umbilical vein endothelial cells were also harvested to determine their gene profile. The study comprised term pregnancies with normal (n = 40) or small-for-gestational-age (n = 20) newborns, small-for-gestational-age preterm pregnancies (n = 15), and bi-chorial, bi-amniotic twin pregnancies with discordant fetal growth (n = 12). Results: Umbilical blood nitrite (p<0.001) and S-nitrosohemoglobin (p = 0.02) rose with fetal growth restriction while asymmetric dimethylarginine decreased (p = 0.003). Nitrite rise coincided with an abnormal Doppler profile from umbilical arteries. Fetal growth restriction umbilical vein endothelial cells produced more nitrite and also exhibited reciprocal changes in vasodilator (upwards) and vasoconstrictor (downwards) transcripts. Elevation in blood nitrite and S-nitrosohemoglobin persisted postnatally in the fetal growth restriction offspring. Conclusion: Fetal growth restriction is typified by increased nitric oxide production during pregnancy and after birth. This response is viewed as an adaptative event to sustain placental blood flow. However, its occurrence may modify the endothelial phenotype and may ultimately represent an element of risk for cardiovascular disease in adult life.Fil: Pisaneschi, Silvia. Università degli Studi di Pisa; Italia. Scuola Superiore Sant’Anna; ItaliaFil: Strigini, Francesca A. L.. Università degli Studi di Pisa; ItaliaFil: Sanchez, Angel Matias. Università degli Studi di Pisa; Italia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; ArgentinaFil: Begliuomini, Silvia. Università degli Studi di Pisa; ItaliaFil: Casarosa, Elena. Università degli Studi di Pisa; ItaliaFil: Ripoli, Andrea. National Research Council. Institute of Clinical Physiology, ; ItaliaFil: Ghirri, Paolo. Università degli Studi di Pisa; ItaliaFil: Boldrini, Antonio. Università degli Studi di Pisa; ItaliaFil: Fink, Bruno. Noxygen Science Transfer and Diagnostics; AlemaniaFil: Genazzani, Andrea R.. Università degli Studi di Pisa; ItaliaFil: Coceani, Flavio. Scuola Superiore Sant’Anna; ItaliaFil: Simoncini, Tommaso. Università degli Studi di Pisa; Itali

Diets supplemented with condensed and hydrolysable tannins affected rumen fatty acid profile and plasmalogen lipids, ammonia and methane production in an in vitro study

The livestock sector constitutes 14.5% of global green-house gas (GHG) emissions and soil and water pollution due to nitrogen excretion. Methane and nitrogen excretions in ruminants can be mitigated by specific feeding strategies, and tannins reduce methanogenesis and ammonia syntheses. In our study, two kinds of condensed tannins (Mimosa and Gambier) and two kinds of hydrolysable tannins (Chestnut and Tara) were added (4 g/100 g DM) to a basal feed (barley: 48 g/100 g DM, wheat bran: 23 g/100 g DM, dehydrated alfalfa hay:15 g/100 g DM, soybean meal: 10 g/100 g DM and molasses: 2 g/100 g DM), inoculated with rumen fluid and fermented for 24 h. The methane, ammonia, fatty acid and plasmalogen lipid profile were determined. The results confirmed that tannins are an important family of heterogeneous compounds whose effect on rumen metabolism is strongly linked to their different characteristics. Chestnut tannin extract was shown to be a good compromise. It improved the sustainability of ruminant rearing by decreasing methanogenesis (control feed 0.159 vs chestnut feed 0.137 mmol/L rumen fluid; p =.0326), ammonia production (control feed 248 vs chestnut feed 179 mg/L rumen fluid; p &lt;.0001) and enhancing acetate synthesis (production rate: chestnut 68.68% vs 49.64% of control).HIGHLIGHTS Tannin extracts from trees and shrubs can be used to modulate rumen fermentation. The positive effect of chestnut tannin extract was demonstrated on methane and ammonia production. Tannins showed no protective action on C18:2 trans 11

Caratteristiche qualitative del latte e del formaggio Caciocavallo nella razza bovina Podolica

Introduction: In recent years the Podolica cattle has increased the demand for dairy products: milk and Caciocavallo cheese are highly appreciated by consumers. In order to high quality product the Podolica breed is a growing interest, it has produces in southern Italian regions as Basilicata. Caciocavallo Silano DOP is one of the 400 cheese varieties produced in Italy, it's produced from "pasta filata". Aim: Aim of the study was analyzed chemical and technological aspects of Podolica milk, moreover fatty acid composition of Caciocavallo cheese was carried out. Materials and methods: The trial was conducted in three farms in province of Potenza, Italy, during two seasons. In winter season when animals were reared in stable and in spring-summer season when cows were reared in outdoor system. During two experimental period milk was sampled from cows in each farm. Samples were analyzed for chemical and technological aspects. The whole of the milk derived from the different farms, during two periods, were used for processing dairy to obtain Caciocavallo Silano DOP cheese in a local dairy. The variance analysis according t-Student test was used to assess statistical differences. Results and discussion: The grazing system during spring and summer improved total fat in milk sampled and increased cheese yield. No difference was observed for technological characteristics, results showed acceptable values in all farms for both farming systems. The fatty acid composition of cheese showed difference in saturated class, mainly for palmitic acid as evinced for stable group that received integration of concentrate. Monounsaturated fatty acid has highlighted significant differences between the experimental groups. Oleic acid C18:1c9 contributes most to the increase of the other also to the total of unsaturated fatty acids. Statistical difference was found for ALA (α-Linolenic acid) a member of the group of essential fatty acids that must be acquired through diet. Conclusion: Different rearing systems led to an improvement of the fat content in the milk and greater cheese yield processing. Pasture group showed a better dietary composition ad showed favorable content specially for ALA. There is some evidence ALA consumption might have a slight preventative effect against cardiovascular diseases. These aspects represented better nutritional quality aspect of Caciocavallo cheese and an economic advantage for breeders

Histochemical characterisation and gene expression analysis of skeletal muscles from maremmana and aubrac steers reared on grazing and feedlot systems

This study aimed to characterise the fibre composition of Triceps brachii (TB) and Semimem-branosus (SM) muscles from 20 Maremmana (MA) and 20 Aubrac (AU) steers, and the effect of grazing activity in comparison with feedlot system. The histochemical method was performed with the m-ATPase method with an acid pre-incubation, thus allowing to distinguish type I, IIA, and IIB fibres. Additionally, on total RNA extracted from SM muscle, the expressions of atp1a1, mt-atp6, and capn1 genes were evaluated, in order to find potential associations with muscle fibre histochemical characteristics. In SM muscle, the MA steers had the greater frequency of oxidative fibres (type I and IIA) and the higher atp1a1 expression, in comparison to AU steers. Conversely, AU steers had a greater frequency of type IIB fibres, and the higher capn1 expression. A similar histochemical pattern was observed in TB muscle. The grazing activity was probably insufficient to determine differences both for fibre proportion and size, and gene expressions, except for mt-atp6 expression that was surprisingly highest in feedlot MA in comparison to other steers. These findings further the knowledge of muscle properties belonging to these breeds, and the effect of voluntary physical activity since few studies were available in this regard

Vertically-Aligned Functionalized Silicon Micropillars for 3D Culture of Human Pluripotent Stem Cell-Derived Cortical Progenitors

Silicon is a promising material for tissue engineering since it allows to produce micropatterned scaffolding structures resembling biological tissues. Using specific fabrication methods, it is possible to build aligned 3D network-like structures. In the present study, we exploited vertically-aligned silicon micropillar arrays as culture systems for human iPSC-derived cortical progenitors. In particular, our aim was to mimic the radially-oriented cortical radial glia fibres that during embryonic development play key roles in controlling the expansion, radial migration and differentiation of cortical progenitors, which are, in turn, pivotal to the establishment of the correct multilayered cerebral cortex structure. Here we show that silicon vertical micropillar arrays efficiently promote expansion and stemness preservation of human cortical progenitors when compared to standard monolayer growth conditions. Furthermore, the vertically-oriented micropillars allow the radial migration distinctive of cortical progenitors in vivo. These results indicate that vertical silicon micropillar arrays can offer an optimal system for human cortical progenitors' growth and migration. Furthermore, similar structures present an attractive platform for cortical tissue engineering